Analysis Note

ControlHepG2 cell lysate

Application

Immunohistochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected MAP3K5 in human pancreas, heart, breast cancer, and prostate tissues.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected MAP3K5 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP3K5 in HeLa cells.

ELISA: A representative lot detected MAP3K5 in ELISA (Direct).

Optimal working dilutions must be determined by end user

Research CategoryApoptosis & Cancer

Anti-MAP3K5 Antibody, clone 2E4 is a highly specific mouse monoclonal antibody, that targets MAP3K5 & has been tested in western blotting, IHC, ICC, Flow Cytometry & ELISA.

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also known as, MAPK/ERK kinase 5 (MEK Kinase 5; MEKK 5) or Apoptosis signal-regulating kinase 1 (ASK-1) protein, is a serine/threonine kinase that is part of the MAP kinase signal transduction pathway. The MAP kinase pathway is central to several signaling cascades the help determine everything from cell fate in differentiation to cell survival in apoptosis. MAP3K5 is critical for innate immune response against numerous pathogens and MAP3K5 also mediates cells responses to various cellular stresses such as oxidative stress, and responses mediated by inflammatory signaling agents such as TNF or LPS. Moreover, MAP3K5 has has been found to be involved in cancer, diabetes, cardiovascular and neurodegenerative diseases. MAP3K5 is membrane associated though not membrane bound directly. Its expression thus is primarily endoplasmic reticulum but can become strongly perinuclear under some conditions. Found in most tissues, it is abundant in heart and pancreas. It′s activity is regulated via phosphorylation-dephosphorylation processes as well as though its interactions with a many other protein partners to form numerous multimolecular cell signaling complexes. EMD-Millipore’s Anti-MAP3K5 mouse monoclonal, clone 2E4, has been tested in western blotting against recombinant human MAP3K5 protein, and in paraffin-embedded immunohistochemistry with human cancer tissues, and in immunofluorescent immunocytochemistry, and flow cytometry on human HeLa cells in culture.

Immunogen

Purified recombinant fragment of human MAP3K5 expressed in E. coli.

Physical form

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MAP3K5 in 10 µg of HepG2 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~155 kDa observed. Uncharacterized bands may appear in some lysate(s).